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Current Edition

December 2016

olume 2  Issue 2

Neonatal Screening and DNA Forensics: An Underappreciated Connection

Michael D. Garrick*

Neonatal screening involves collecting dried blood spots on filter paper from essentially all newborns in most countries. These specimens are a potential store of each child’s DNA. In some locales, laboratories have stored these spots for 50 years. There are risks and benefits associated with having these specimens in storage. This opinion article argues that now is the time to weigh the balance for keeping such spots in long term storage and choose to do so in a fashion that preserves the rights and privacy of the DNA’s source or decide not to keep the specimens indefinitely and choose when to discard them.

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Case Report 

A Case-Report of Chemical Pneumonitis and Burns from Pressurized Liquid Ammonia

Maria Stefanidou*, Sotiris Athanaselis, Panagiota Nikolaou, Ioannis Papoutsis, Ioanna Vardakou, Ioannis Fountos, Julia  Atta-Politou, Chara Spiliopoulou, Stavroula Papadodima

Anhydrous ammonia is widely used in industry and agriculture. Therefore, accidental exposure to it is relatively common. The autopsy findings of a 35-year-old woman who died shortly after exposure to anhydrous pressurized liquid ammonia during an industrial accident is presented. The victim showed chemical burns on her body surface and on her face, conjunctivitis and opaque cornea on both eyes, as well as pulmonary oedema and congested lungs with large areas of hemorrhage. These findings were consistent with injuries inflicted by a corrosive substance like ammonia. Such accidents can be prevented. The use of protective equipment should be mandatory and workplace physicians should be aware of the possible dangers of handling pressurized anhydrous ammonia and educate accordingly the workers.               

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Orignal Research

Differential Extraction of Sperm Mixtures Deconvolutes the Mixture

Lay-Hong Seah*, Boon-Hui Wee

The differential extraction procedure first described by Gill et al. refers to the process by which the DNA from epithelial cells and sperm cells can be separated into different fractions. Compared with other methods such as flow cytometry  and laser microdissection, differential extraction is relatively quick, effective and low-cost . In the investigation of cases of sexual assault, semen stains on vaginal swabs and clothing or bedding items provide the most incriminating evidence.               

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